CRP-sante Microscopy Imaging Facilities (CMIF)

Users

New users will get an introduction to the equipment.
Inexperienced users will be trained before access to the equipment.
Feel free to contact me for any help or advice to start your microscopy experiment.


Booking for CRP-Santé users: via LotusNotes

Go to 'calendar' and 'Schedule a meeting'
In where: select 'resources' and 'CMIF-microscope confocal'; clic on 'add' and 'OK'
In scheduler: 'click to see resource availability'; to see the details and booked peoples, put the mouse on the ressource name and clic right to 'open calendar', select the confocal in 'view by room or resource'
In when: select your booking zone time.
In subject: put your name, lab and phone number
 

Booking for external users:

contact: Dr Hoffmann Céline
00 352 26970-253
celine.hoffmann@crp-sante.lu


Handbooks:  Illustrated powerpoint presentation with step by step description of the use of the Axiovert microscope and the LSM 510 software are available on the intranet. 


General information:

 - Remember to fill the logbook on the intranet, the application is called microscopy: date, name, time etc, when you start and when you stop.

 - Immersion oil can damage the inverted objectives, always clean the objectives after use and clean immediately if you accidentally immerse the water objective (cleaning solution: ether petroleum 95%, isopropyl-alcohol 5%).

  - When you stop :
If the next user comes in less than one hour, leave the system on, put the laser argon on standby; but make sure the next person will come.
If the next user has booked for later than in one hour, shut down the system.

- For data-storage, put your data on the the server or take a CD or USB key for your personal backup.

 - To view your pictures, you can download the Zeiss LSM image browser, http://www.zeiss.fr/C12568E80022E0FD/?Open (In "téléchargements", "logiciel imagerie").

- Samples can be observed on microscope slides as well as in chambers (25 or 42 mm diameter), in Petri dishes or multiwell plates, but you have to use glass bottom dishes for high magnification or Long Distance objectives for plastic dishes.

- Please ask if you have any doubt how to operate the microscope.