CRP-Santé Microscopy Imaging Facilities (CMIF)

Equipment                                                  Room: 1-08 1st floor BAM       Phone: 26970-293




The Microscope: Axiovert 200M



- The microscope is an inverted Axiovert 200M from Carl      Zeiss.

- The system is available with heating and CO2  control  
   for timelapse experiments.

 
- The illumination light is a LED system with 3 LED;
    365nm, 470nm and 555 nm.


- The camera is a monochromatic Axiocam MRm.



Filters:

Filters Set01 (DAPI) Set 09 (GFP-LP) Set 15 (TRITC) Set 38 (GFP-BP)
Excitation BP 365/12 BP 450-490 BP 546/12 BP 450-490
Emission LP 397 LP 515 LP 590 BP 500-550



Objectives available:

magnification type NA contrast immersion WD (working distance)
x10 Plan NeoFluar 0.3 - - 5.6 mm
x10 A-Plan 0.25 PlasDIC - 4.4 mm
x20 Plan Neofluar 0.5 DIC II - 2 mm
x20 LD A-Plan
0.3 PlasDIC - 4.3 mm
x40 LD Plan Neofluar 0.6 Phase - 2.9 mm
x40 Plan Neofluar 1.3 DIC III oil 0.2 mm
x63 LD Plan Neofluar 0.75 Phase - 1.7 mm
x63 Plan Apochromat 1.4 DIC III oil 0.18 mm
x63 C-Apochromat 1.2 DIC III water 0.24 mm
x100 EC Plan Neofluar 1.3 Phase oil 0.2 mm